Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis with contactless conductivity and ultraviolet photometric detection-An efficient tool for monitoring of inborn metabolic disorders

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10104480" target="_blank" >RIV/00216208:11310/11:10104480 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/11:00002418
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2010.11.007" target="_blank" >http://dx.doi.org/10.1016/j.aca.2010.11.007</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2010.11.007" target="_blank" >10.1016/j.aca.2010.11.007</a>

Result language
angličtina
Original language name
Determination of the spectrum of low molecular mass organic acids in urine by capillary electrophoresis with contactless conductivity and ultraviolet photometric detection-An efficient tool for monitoring of inborn metabolic disorders
Original language description
A mixture of 29 organic acids (OAs) occurring in urine was analyzed by capillary electrophoresis (CE) with capacitively coupled contactless conductivity detection (C4D) and UV photometric detection. The optimized analytical system involved a 100 cm longpolyacrylamide-coated capillary (50 ?m i.d.) and the background electrolyte of 20 mM 2-morpholinoethanesulfonic acid (MES)/NaOH + 10% (v/v) methanol, pH 6.0 (pH is related to the 20 mM MES/NaOH buffer in water). The LOD values obtained by C4D for the OAswhich do not absorb UV radiation range from 0.6 ?M (oxalic acid) to 6.8 ?M (pyruvic acid); those obtained by UV photometry for the remaining OAs range from 2.9 ?M (5-hydroxy-3-indoleacetic acid) to 10.2 ?M (uric acid). The repeatability of the proceduredeveloped is characterized by the coefficients of variation, which vary between 0.3% (tartaric acid) and 0.6% (5-hydroxy-3-indoleacetic acid) for the migration time and between 1.3% (tartaric acid) and 3.5% (lactic acid) for the peak are
Czech name
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Czech description
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Project
<a href="/en/project/GAP206%2F10%2F1231" target="_blank" >GAP206/10/1231: New Electrophoretic Approaches to the Monitoring of Pain Neurotransmitters in the Brain Tissue of Higher Organisms</a>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP) Z - Vyzkumny zamer (s odkazem do CEZ) I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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