Stress-induced expression of p53 target genes is insensitive to SNW1/SKIP downregulation

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10104653" target="_blank" >RIV/00216208:11310/11:10104653 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2478/s11658-011-0012-1" target="_blank" >http://dx.doi.org/10.2478/s11658-011-0012-1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11658-011-0012-1" target="_blank" >10.2478/s11658-011-0012-1</a>

Result language

angličtina

Original language name

Stress-induced expression of p53 target genes is insensitive to SNW1/SKIP downregulation

Original language description

Pharmacological inhibition of protein kinases that are responsible for the phosphorylation of the carboxy-terminal domain (CTD) of RNA Pol II during transcription by 5,6-dichloro-1-beta-D-ribofuranosyl-benzimidazole (DRB) leads to severe inhibition of mRNA synthesis and activates p53. Transcription of the p53 effectors that are induced under these conditions, such as p21 or PUMA, must bypass the requirement for CTD phosphorylation by the positive elongation factor P-TEFb. Here, we have downregulated SNW1/SKIP, a splicing factor and a transcriptional co-regulator, which was found to interact with P-TEFb and synergistically affect Tat-dependent transcription elongation of HIV 1. Using the colon cancer derived cell line HCT116, we have found that both doxorubicin- and DRB-induced expression of p21 or PUMA is insensitive to SNW1 downregulation by siRNA. This suggests that transcription of stress response genes, unlike, e.g., the SNW1-sensitive mitosis-specific genes, can proceed uncoupled

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/LC06061" target="_blank" >LC06061: Center of Cell Invasiveness in Embryonic Development and Tumour Metastases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular and Molecular Biology Letters

ISSN

1425-8153

e-ISSN

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Volume of the periodical

16

Issue of the periodical within the volume

3

Country of publishing house

PL - POLAND

Number of pages

12

Pages from-to

373-384

UT code for WoS article

000293019200002

EID of the result in the Scopus database

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