Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10108255" target="_blank" >RIV/00216208:11310/11:10108255 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/11:00360800
Result on the web
<a href="http://dx.doi.org/10.1016/j.procbio.2010.10.004" target="_blank" >http://dx.doi.org/10.1016/j.procbio.2010.10.004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.procbio.2010.10.004" target="_blank" >10.1016/j.procbio.2010.10.004</a>
Alternative languages
Result language
angličtina
Original language name
Production, enrichment and immobilization of a metagenome-derived epoxide hydrolase
Original language description
Optimised culture conditions for the recombinant prototrophic strain Escherichia coli RE3(pSEKau2) were established to obtain biomass of epoxide hydrolase (EH). The recombinant enzyme was extracted from crude extracts using an optimised polyethylene glycol-based aqueous two-phase system (ATPS). Eupergit C 250L carrier proved to be the most interesting in terms of partially purified EH immobilization. The immobilized EH exhibited a significantly higher thermal stability compared to the free enzyme. Immobilization did not alter, to a large extent, the enantioselectivity and regioselectivity of the enzyme
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Process Biochemistry
ISSN
1359-5113
e-ISSN
—
Volume of the periodical
46
Issue of the periodical within the volume
2
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
526-532
UT code for WoS article
000287274000015
EID of the result in the Scopus database
—