Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F11%3A10118931" target="_blank" >RIV/00216208:11310/11:10118931 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.2478/s11532-011-0062-2" target="_blank" >http://dx.doi.org/10.2478/s11532-011-0062-2</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2478/s11532-011-0062-2" target="_blank" >10.2478/s11532-011-0062-2</a>

Result language

angličtina

Original language name

Determination of N-methyl-2-pyrrolidone and its metabolites in urine by micellar electrokinetic chromatography

Original language description

A fast and accurate micellar electrokinetic capillary chromatography (MEKC) method was developed for monitoring N-methyl-2-pyrrolidone (NMP) exposure. Baseline separation of NMP and its main metabolites: 5-hydroxy-N-methyl-2-pyrrolidone (5HNMP), N-methylsuccinimide (MSI), 2-hydroxy-N-methylsuccinimide (2HMSI), and 2-pyrrolidone (2P) was obtained within 6 min in an uncoated fused silica capillary using 5 mM phosphate buffer and 140 mM sodium dodecyl sulfate (pH 7.1) as background electrolyte (BGE). On-line UV-detection was performed at 200 nm and the applied electric field was 400 V cm(-1). Possible interference of BGE-induced system peaks on separation was investigated by computer simulation and no such interference was observed. The developed MEKC method combined with solid phase extraction for sample preparation was successfully applied to the analysis of urine of rats exposed to NMP. The urinary excretion was determined in 0-6 h and 6-24 h specimens collected after an intragastic ad

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Central European Journal of Chemistry

ISSN

1895-1066

e-ISSN

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Volume of the periodical

9

Issue of the periodical within the volume

5

Country of publishing house

PL - POLAND

Number of pages

9

Pages from-to

825-833

UT code for WoS article

000293020400008

EID of the result in the Scopus database

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