Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10105630" target="_blank" >RIV/00216208:11310/12:10105630 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/12:43908631
Result on the web
<a href="http://www.springerlink.com/content/31407v4281424368/" target="_blank" >http://www.springerlink.com/content/31407v4281424368/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s11627-011-9386-z" target="_blank" >10.1007/s11627-011-9386-z</a>
Alternative languages
Result language
angličtina
Original language name
Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)
Original language description
Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway ( Carum carvi L.). Microspore- and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donorplants (2 n = 20) and their anther-derived derivative plants ( n = 10, 2 n = 20, 4 n = 40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parenta
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
In Vitro Cellular and Developmental Biology - Plant
ISSN
1054-5476
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
30-39
UT code for WoS article
000300330000005
EID of the result in the Scopus database
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