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Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10105630" target="_blank" >RIV/00216208:11310/12:10105630 - isvavai.cz</a>

  • Alternative codes found

    RIV/62156489:43210/12:43908631

  • Result on the web

    <a href="http://www.springerlink.com/content/31407v4281424368/" target="_blank" >http://www.springerlink.com/content/31407v4281424368/</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/s11627-011-9386-z" target="_blank" >10.1007/s11627-011-9386-z</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Induction conditions for somatic and microspore-derived structures and detection of haploid status by isozyme analysis in anther culture of caraway (Carum carvi; L.)

  • Original language description

    Plant regeneration was obtained from cultured anthers and hypocotyl segments of caraway ( Carum carvi L.). Microspore- and somatic tissue-derived embryos were compared by observation of the regeneration process under identical induction conditions. Fluorescent microscopy with DAPI staining showed initiation of cell divisions and formation of embryogenic callus and somatic embryos from anther sacs, with production of embryos of both microspore and somatic origin. Induction of somatic embryos from hypocotyl-derived callus was also demonstrated. Isozyme native polyacrylamide gel electrophoresis was used to identify haploids and doubled haploids, and to determine the frequency of spontaneous diploidization of regenerated plants of microspore origin. Donorplants (2 n = 20) and their anther-derived derivative plants ( n = 10, 2 n = 20, 4 n = 40) in callus stage or leafy rosette stage were compared. The esterase (EST) band patterns of regenerated plants differed from the heterozygous parenta

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

  • CEP classification

    EF - Botany

  • OECD FORD branch

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2012

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    In Vitro Cellular and Developmental Biology - Plant

  • ISSN

    1054-5476

  • e-ISSN

  • Volume of the periodical

    48

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    10

  • Pages from-to

    30-39

  • UT code for WoS article

    000300330000005

  • EID of the result in the Scopus database