Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10124052" target="_blank" >RIV/00216208:11310/12:10124052 - isvavai.cz</a>
Alternative codes found
RIV/68081707:_____/12:00382138 RIV/61388963:_____/12:00382138 RIV/00216224:14740/12:00073028
Result on the web
<a href="http://dx.doi.org/10.1002/cplu.201200056" target="_blank" >http://dx.doi.org/10.1002/cplu.201200056</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cplu.201200056" target="_blank" >10.1002/cplu.201200056</a>
Alternative languages
Result language
angličtina
Original language name
Synthesis of Hydrazone-Modified Nucleotides and Their Polymerase Incorporation onto DNA for Redox Labeling
Original language description
5-(5-Formylthiophen-2-yl)cytosine and -7-deazaadenosine 2'-deoxyribonucleosides and their triphosphates were converted to the 2,4-dinitrophenyl- or nitrobenzofurazanyl hydrazones. The hydrazone-modified dNTPs were enzymatically incorporated to DNA by polymerase catalyzed primer extension. This direct incorporation of hydrazone-linked dNTPs was compared to previously reported incorporation of aldehyde-modified dNTPs followed by post-synthetic hydrazone-formation on DNA. Electrochemical study the nitroarylhydrazone-linked nucleosides and DNA was performed confirming the potential of the hydrazones, nitroaryls and benzofurazane groups for redox labelling of DNA.
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CC - Organic chemistry
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
ChemPlusChem [online]
ISSN
2192-6506
e-ISSN
—
Volume of the periodical
77
Issue of the periodical within the volume
8
Country of publishing house
DE - GERMANY
Number of pages
11
Pages from-to
652-662
UT code for WoS article
000307401800006
EID of the result in the Scopus database
—