Microscale separation methods for enzyme kinetics assays

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10124388" target="_blank" >RIV/00216208:11310/12:10124388 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00216-012-5744-x" target="_blank" >http://dx.doi.org/10.1007/s00216-012-5744-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00216-012-5744-x" target="_blank" >10.1007/s00216-012-5744-x</a>

Result language

angličtina

Original language name

Microscale separation methods for enzyme kinetics assays

Original language description

Miniaturization continues to be one of the leading trends in analytical chemistry and one that brings advantages that can be particularly beneficial in biochemical research. Use of a miniaturized scale enables efficient analysis in a short time and requires very small amounts of samples, solvents, and reagents. This can result in a remarkable decrease in costs of enzyme kinetics studies, especially when expensive or rare enzymes and/or substrates are involved. Free zone electrophoresis is without a doubt the most common microscale separation technique for capillary and on-chip enzyme assays. Progress and applications in this field are reviewed frequently whereas other modes of separation, although successfully applied, receive only marginal interest insuch publications. This review summarizes applications of less common modes of separation in capillary or chip formats, namely micellar electrokinetic chromatography, liquid chromatography, gel electrophoresis, isoelectric focusing, and

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Analytical and Bioanalytical Chemistry

ISSN

1618-2642

e-ISSN

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Volume of the periodical

403

Issue of the periodical within the volume

8

Country of publishing house

DE - GERMANY

Number of pages

11

Pages from-to

2185-2195

UT code for WoS article

000305127200011

EID of the result in the Scopus database

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