Production of Aspergillus niger beta-mannosidase in Pichia pastoris

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10125394" target="_blank" >RIV/00216208:11310/12:10125394 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/12:00383469
Result on the web
<a href="http://dx.doi.org/10.1016/j.pep.2012.07.012" target="_blank" >http://dx.doi.org/10.1016/j.pep.2012.07.012</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.pep.2012.07.012" target="_blank" >10.1016/j.pep.2012.07.012</a>

Result language
angličtina
Original language name
Production of Aspergillus niger beta-mannosidase in Pichia pastoris
Original language description
beta-Mannosidase (EC 3.2.1.25) is an exoglycosidase specific for the hydrolysis of terminal beta-linked mannoside in various sugar chains. cDNA corresponding to the beta-mannosidase gene was cloned from Aspergillus niger, sequenced, and expressed in theyeast Pichia pastoris. The beta-mannosidase gene contains an open reading frame which encodes the protein with 933 amino acid residues. The wild type and recombinant proteins were purified to apparent homogeneity and biochemically characterized (K-M 0.28and 0.44 mmol/l for p-nitrophenyl beta-D-mannopyranoside, pI 4.2 and 4.0, and their pH optima were at pH 4.5 and 5.5 and 65 degrees C, respectively).
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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