A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae From Bee Debris

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10129223" target="_blank" >RIV/00216208:11310/12:10129223 - isvavai.cz</a>
Alternative codes found
RIV/67179843:_____/12:00384289
Result on the web
<a href="http://www.bioone.org/doi/abs/10.1603/EC12010" target="_blank" >http://www.bioone.org/doi/abs/10.1603/EC12010</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1603/EC12010" target="_blank" >10.1603/EC12010</a>

Result language
angličtina
Original language name
A New Low-Cost Procedure for Detecting Nucleic Acids in Low-Incidence Samples: A Case Study of Detecting Spores of Paenibacillus larvae From Bee Debris
Original language description
American foulbrood, because of its virulence and worldwide spread, is currently one of the most dangerous diseases of honey bees. Quick diagnosis of this disease is therefore vitally important. For its successful eradication, however, all the hives in the region must be tested. This is time consuming and costly. Therefore, a fast and sensitive method of detecting American foulbrood is needed. Here we present a method that significantly reduces the number of tests needed by combining batches of samples from different hives. The results of this method were verified by testing each sample. A simulation study was used to compare the efficiency of the new method with testing all the samples and to develop a decision tool for determining when best to use thenew method. The method is suitable for testing large numbers of samples (over 100) when the incidence of the disease is low (10% or less)
Czech name
—
Czech description
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Project
<a href="/en/project/SP%2F2D3%2F60%2F08" target="_blank" >SP/2D3/60/08: Integrated research of the effects and consequences of artificially reared mallards (Anas platyrhynchos) on wetland ecosystems with the application of advanced ecological and molecular techniques.</a>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP) Z - Vyzkumny zamer (s odkazem do CEZ) I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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