Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F12%3A10132173" target="_blank" >RIV/00216208:11310/12:10132173 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/12:00381075
Result on the web
<a href="http://www.amjbot.org/content/99/2/e74.full.pdf+html" target="_blank" >http://www.amjbot.org/content/99/2/e74.full.pdf+html</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3732/ajb.1100416" target="_blank" >10.3732/ajb.1100416</a>

Result language
angličtina
Original language name
Development of novel low-copy nuclear markers for Hieraciinae (Asteraceae) and their perspective for other tribes
Original language description
The development of three low-copy nuclear markers for low taxonomic level phylogenies in Asteraceae with emphasis on the subtribe Hieraciinae is reported. Marker candidates were selected by comparing a Lactuca complementary DNA (cDNA) library with publicDNA sequence databases. Interspecifi c variation and phylogenetic signal of the selected genes were investigated for diploid taxa from the subtribe Hieraciinae and compared to a reference phylogeny. Their ability to cross-amplify was assessed for otherAsteraceae tribes. All three markers had higher variation (2.1 MINUS SIGN 4.5 times) than the internal transcribed spacer (ITS) in Hieraciinae. Cross-amplifi cation was successful in at least seven other tribes of the Asteraceae. Only three cases indicating the presence of paralogs or pseudogenes were detected. The results demonstrate the potential of these markers for phylogeny reconstruction in the Hieraciinae as well as in other Asteraceae tribes, esp. for very closely related species
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2012
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)