Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10133399" target="_blank" >RIV/00216208:11310/13:10133399 - isvavai.cz</a>

Alternative codes found

RIV/61989592:15110/13:33143419

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Keratin Cross-reactivity in Electro-transfer (Western blot): How to Suppress It

Original language description

Chicken antibodies (IgY) are increasingly being used as a primary antibody in the Western blotting immunodetection, offering several advantages compared to the common mammalian antibodies. The presence of additional bands in the region of 45-70 kDa, vertical streaks, and spots on the membrane, caused by human keratins, made the evaluation of the developed blots hardly possible. In this study, we describe a method how to eliminate the cross-reactivity of IgY with human keratins.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CG - Electrochemistry

OECD FORD branch

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Project

<a href="/en/project/GBP303%2F12%2FG163" target="_blank" >GBP303/12/G163: Centre of drug-dietary supplements interactions and nutrigenetics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

International Journal of Electrochemical Science

ISSN

1452-3981

e-ISSN

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Volume of the periodical

8

Issue of the periodical within the volume

2

Country of publishing house

RS - THE REPUBLIC OF SERBIA

Number of pages

8

Pages from-to

1551-1558

UT code for WoS article

000316565800001

EID of the result in the Scopus database

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