Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10134468" target="_blank" >RIV/00216208:11310/13:10134468 - isvavai.cz</a>
Alternative codes found
RIV/62156489:43210/13:00193682
Result on the web
<a href="http://www.electrochemsci.org/papers/vol8/80100125.pdf" target="_blank" >http://www.electrochemsci.org/papers/vol8/80100125.pdf</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
Photo-cytochrome b(5) - A New Tool to Study the Cytochrome P450 Electron-transport Chain
Original language description
To study the enigmatic role of cytochrome b(5) in a cytochrome P450 monooxygenase multienzyme system, we expressed and purified a photoreactive analogue of cytochrome b(5), having methionine residues substituted with photolabile diazirine derivatives (photo-cyt b(5)). An equimolar mixture of cytochrome P450 (CYP) 2B4 and photo-cyt b(5) was photolyzed and products separated using SDS-PAGE. Several oligomers composed of CYP2B4 and cyt b(5) (MALDI-TOF/TOF mass spectrometry) in 1: 1, 1: 2, and 2: 1 molar ratios have been found and their covalent nature confirmed by MALDI-TOF/TOF mass spectrometry of chymotryptic peptides. The amount of oligomers formed was markedly increased by the presence of the CYP2B4 substrate, diamantane, showing that the substrate binding increases the affinity between cytochromes P450 and b(5).
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal of Electrochemical Science
ISSN
1452-3981
e-ISSN
—
Volume of the periodical
8
Issue of the periodical within the volume
1
Country of publishing house
RS - THE REPUBLIC OF SERBIA
Number of pages
10
Pages from-to
125-134
UT code for WoS article
000316562800010
EID of the result in the Scopus database
—