Detailed kinetic analysis of the interaction between the FOXO4-DNA-binding domain and DNA
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10139882" target="_blank" >RIV/00216208:11310/13:10139882 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/13:00397654 RIV/61388971:_____/13:00397654 RIV/00216208:11320/13:10139882
Result on the web
<a href="http://dx.doi.org/10.1016/j.bpc.2013.09.002" target="_blank" >http://dx.doi.org/10.1016/j.bpc.2013.09.002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2013.09.002" target="_blank" >10.1016/j.bpc.2013.09.002</a>
Alternative languages
Result language
angličtina
Original language name
Detailed kinetic analysis of the interaction between the FOXO4-DNA-binding domain and DNA
Original language description
In this work, the real-time kinetics of the interaction between the FOXO4-DNA binding domain (FOXO4-DBD) and the DNA was studied by using surface plasmon resonance (SPR). SPR analysis revealed that the interaction between FOXO4-DBD and the double stranded DNA containing either the insulin-responsive or the Daf-16 family member-binding element is preferably described by using a conformational change model which suggests a structural change of FOXO4-DBD upon binding to the DNA. Alanine scanning of amino acid residues engaged in polar contacts with the DNA showed that certain non-specific contacts with the DNA backbone are very important for both the binding affinity and the binding specificity of FOXO4-DBD
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biophysical Chemistry
ISSN
0301-4622
e-ISSN
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Volume of the periodical
184
Issue of the periodical within the volume
December
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
68-78
UT code for WoS article
000328443300010
EID of the result in the Scopus database
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