The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10189519" target="_blank" >RIV/00216208:11310/13:10189519 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1017/S0031182013000358" target="_blank" >http://dx.doi.org/10.1017/S0031182013000358</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1017/S0031182013000358" target="_blank" >10.1017/S0031182013000358</a>
Alternative languages
Result language
angličtina
Original language name
The role of surface glycoconjugates in Leishmania midgut attachment examined by competitive binding assays and experimental development in sand flies
Original language description
Binding of promastigotes to the sand fly midgut epithelium is regarded as an essential part of the Leishmania life cycle in the vector. Among Leishmania surface molecules putatively involved in attachment to the sand fly midgut, two GPI-anchored molecules are the most prominent: lipophosphoglycan (LPG) and promastigote surface protease gp63. In this work, we examined midgut attachment of Leishmania lines mutated in GPI-anchored molecules and compared results from 2 different techniques: in vivo development in sand flies and in vitro competitive binding assays using fluorescently labelled parasites. In combination with previous studies, our data provide additional support for (1) an LPG-independent parasite-binding mechanism of Leishmania major within the midgut of the permissive vector Phlebotomus perniciosus, and provide strong support for (2) the crucial role of L. major LPG in specific vector Phlebotomus papatasi, and (3) a role for Leishmania amazonensis gp63 in Lutzomyia longipalp
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Parasitology
ISSN
0031-1820
e-ISSN
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Volume of the periodical
140
Issue of the periodical within the volume
8
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1026-1032
UT code for WoS article
000320035100010
EID of the result in the Scopus database
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