Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10190704" target="_blank" >RIV/00216208:11310/13:10190704 - isvavai.cz</a>
Alternative codes found
RIV/61389030:_____/13:00398129
Result on the web
<a href="http://dx.doi.org/10.1091/mbc.E12-06-0492" target="_blank" >http://dx.doi.org/10.1091/mbc.E12-06-0492</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1091/mbc.E12-06-0492" target="_blank" >10.1091/mbc.E12-06-0492</a>
Alternative languages
Result language
angličtina
Original language name
Visualization of the exocyst complex dynamics at the plasma membrane of Arabidopsis thaliana
Original language description
The exocyst complex, an effector of Rho and Rab GTPases, is believed to function as an exocytotic vesicle tether at the plasma membrane before soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) complex formation. Exocyst subunits localize to secretory-active regions of the plasma membrane, exemplified by the outer domain of Arabidopsis root epidermal cells. Using variable-angle epifluorescence microscopy, we visualized the dynamics of exocyst subunits at this domain. The subunits colocalized in defined foci at the plasma membrane, distinct from endocytic sites. Exocyst foci were independent of cytoskeleton, although prolonged actin disruption led to changes in exocyst localization. Exocyst foci partially overlapped with vesicles visualized by VAMP721 v-SNARE, but the majority of the foci represent sites without vesicles, as indicated by electron microscopy and drug treatments, supporting the concept of the exocyst functioning as a dynamic particle. We observe
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
ED - Physiology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP305%2F11%2F1629" target="_blank" >GAP305/11/1629: Functions of the plant exocyst tethering complex in exocytosis, cell division and cell wall biogenesis.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular Biology of the Cell
ISSN
1059-1524
e-ISSN
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Volume of the periodical
24
Issue of the periodical within the volume
4
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
510-520
UT code for WoS article
000321117800008
EID of the result in the Scopus database
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