Structural Study of the Partially Disordered Full-Length delta Subunit of RNA Polymerase from Bacillus subtilis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191385" target="_blank" >RIV/00216208:11310/13:10191385 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/13:00422985 RIV/00216224:14740/13:00066324
Result on the web
<a href="http://dx.doi.org/10.1002/cbic.201300226" target="_blank" >http://dx.doi.org/10.1002/cbic.201300226</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cbic.201300226" target="_blank" >10.1002/cbic.201300226</a>

Result language
angličtina
Original language name
Structural Study of the Partially Disordered Full-Length delta Subunit of RNA Polymerase from Bacillus subtilis
Original language description
The partially disordered delta subunit of RNA polymerase was studied by various NMR techniques. The structure of the well-folded N-terminal domain was determined based on inter-proton distances in NOESY spectra. The obtained structural model was comparedto the previously determined structure of a truncated construct (lacking the C-terminal domain). Only marginal differences were identified, thus indicating that the first structural model was not significantly compromised by the absence of the C-terminal domain. Various N-15 relaxation experiments were employed to describe the flexibility of both domains. The relaxation data revealed that the C-terminal domain is more flexible, but its flexibility is not uniform. By using paramagnetic labels, transientcontacts of the C-terminal tail with the N-terminal domain and with itself were identified. A propensity of the C-terminal domain to form beta-type structures was obtained by chemical shift analysis. Comparison with the paramagnetic rela
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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