CYCLONE-A Utility for De Novo Sequencing of Microbial Cyclic Peptides

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191628" target="_blank" >RIV/00216208:11310/13:10191628 - isvavai.cz</a>

Alternative codes found

RIV/61388971:_____/13:00399080

Result on the web

<a href="http://dx.doi.org/10.1007/s13361-013-0652-7" target="_blank" >http://dx.doi.org/10.1007/s13361-013-0652-7</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s13361-013-0652-7" target="_blank" >10.1007/s13361-013-0652-7</a>

Result language

angličtina

Original language name

CYCLONE-A Utility for De Novo Sequencing of Microbial Cyclic Peptides

Original language description

We have developed a de novo sequencing software tool (CYCLONE) and applied it for determination of cyclic peptides. The program uses a non-redundant database of 312 nonribosomal building blocks identified to date in bacteria and fungi (more than 230 additional residues in the database list were isobaric). The software was used to fully characterize the tandem mass spectrum of several cyclic peptides and provide sequence tags. The general strategy of the script was based on fragment ion pre-characterization to accomplish unambiguous b-ion series assignments. Showcase examples were a cyclic tetradepsipeptide beauverolide, a cyclic hexadepsipeptide roseotoxin A, a lasso-like hexapeptide pseudacyclin A, and a cyclic undecapeptide cyclosporin A. The extentof ion scrambling in smaller peptides was as low as 5 % of total ion current; this demonstrated the feasibility of CYCLONE de novo sequencing. The robustness of the script was also tested against database sets of various sizes and isotope

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of the American Society for Mass Spectrometry

ISSN

1044-0305

e-ISSN

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Volume of the periodical

24

Issue of the periodical within the volume

8

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

8

Pages from-to

1177-1184

UT code for WoS article

000321923100004

EID of the result in the Scopus database

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