In vivo exposure to 17 beta-estradiol triggers premature sperm capacitation in cauda epididymis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10191736" target="_blank" >RIV/00216208:11310/13:10191736 - isvavai.cz</a>
Alternative codes found
RIV/86652036:_____/13:00388348
Result on the web
<a href="http://dx.doi.org/10.1530/REP-12-0472" target="_blank" >http://dx.doi.org/10.1530/REP-12-0472</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1530/REP-12-0472" target="_blank" >10.1530/REP-12-0472</a>
Alternative languages
Result language
angličtina
Original language name
In vivo exposure to 17 beta-estradiol triggers premature sperm capacitation in cauda epididymis
Original language description
Estrogens play a crucial role in spermatogenesis and estrogen receptor a knock-out male mice are infertile. It has been demonstrated that estrogens significantly increase the speed of capacitation in vitro; however this may lead to the reduction of reproductive potential due to the decreased ability of these sperm to undergo the acrosome reaction. To date the in vivo effect of estrogens on the ability of sperm to capacitate has not been investigated. Therefore, in this study, we exposed mice (n=24) to 17 beta-estradiol (E-2) at the concentration of 20 ng/ml either during puberty from the fourth to seventh week of age (n=8), or continuously from birth for a period of 12 weeks (n=8) at which age the animals from both groups were killed. The capacitationstatus of epididymal and testicular sperm was analysed by tyrosine phosphorylation (TyrP) antibody (immunofluorescence and western blot) and chlortetracycline (CTC) assay. According to our results, in vivo exposure to increased E-2 concen
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Reproduction
ISSN
1470-1626
e-ISSN
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Volume of the periodical
145
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
9
Pages from-to
255-263
UT code for WoS article
000317562900007
EID of the result in the Scopus database
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