The Luc2 gene enhances reliability of bicistronic assays (vol 8, pg 423, 2013)

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F13%3A10192465" target="_blank" >RIV/00216208:11310/13:10192465 - isvavai.cz</a>
Result on the web
<a href="http://link.springer.com/article/10.2478%2Fs11535-013-0151-z#page-1" target="_blank" >http://link.springer.com/article/10.2478%2Fs11535-013-0151-z#page-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11535-013-0151-z" target="_blank" >10.2478/s11535-013-0151-z</a>

Result language
angličtina
Original language name
The Luc2 gene enhances reliability of bicistronic assays (vol 8, pg 423, 2013)
Original language description
Luciferases are prominent reporters in molecular and cellular biology investigations including miRNA target studies and the determination of Internal Ribosome Entry Site (IRES) activities in bicistronic assays. A majority of the current bicistronic vectors contain a firefly luciferase reporter as the second cistron. One reason for this is the presence of cryptic transcription start sites inside the luciferase gene. We present here an experimental evaluation of the cryptic transcription within the latestversion of the firefly luciferase gene, luc2. Using flow cytometric analysis, we observed a negligible amount of cryptic transcriptional activity that was only slightly above the background of untransfected cells. Nevertheless, quantitative reverse transcription PCR experiments revealed a six-to-nine-fold gradual increase of transcription along the coding region of the gene. The level of cryptic transcription from the coding region of the improved luc2 firefly luciferase gene is signifi
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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