DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10210464" target="_blank" >RIV/00216208:11310/14:10210464 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.2478/s11532-014-0516-4" target="_blank" >http://dx.doi.org/10.2478/s11532-014-0516-4</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.2478/s11532-014-0516-4" target="_blank" >10.2478/s11532-014-0516-4</a>

Result language
angličtina
Original language name
DNA-based biosensors with external Nafion and chitosan membranes for the evaluation of the antioxidant activity of beer, coffee, and tea
Original language description
Novel electrochemical DNA-based biosensors with outer-sphere Nafion and chitosan protective membranes were prepared for the evaluation of antioxidant properties of beverages (beer, coffee, and black tea) against prooxidant hydroxyl radicals. A carbon working electrode of a screen-printed three-electrode assembly was modified using a layer-by-layer deposition technique with low molecular weight double-stranded DNA and a Nafion or chitosan film. The membrane-covered DNA biosensors were initially tested with respect to their voltammetric and impedimetric response after the incubation of the beverage and the medium exchange for the solution of the redox indicator [Fe(CN)(6)](3-/4-). While the Nafion-protected biosensor proved to be suitable for beer and black tea extracts, the chitosan-protected biosensor was successfully used in a coffee extract. Afterwards, the applicability was successfully verified for these biosensors for the detection of a deep degradation of the surface-attached DNA
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CG - Electrochemistry
OECD FORD branch
—

Project
<a href="/en/project/GP13-23337P" target="_blank" >GP13-23337P: Complex diagnostic approach in the evaluation of the interaction of DNA with organic xenobiotics using electrochemical nanostructured DNA biosensors</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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