CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10210779" target="_blank" >RIV/00216208:11310/14:10210779 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1007/s00018-013-1450-x" target="_blank" >http://dx.doi.org/10.1007/s00018-013-1450-x</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s00018-013-1450-x" target="_blank" >10.1007/s00018-013-1450-x</a>

Result language

angličtina

Original language name

CAS directly interacts with vinculin to control mechanosensing and focal adhesion dynamics

Original language description

Focal adhesions are cellular structures through which both mechanical forces and regulatory signals are transmitted. Two focal adhesion-associated proteins, Crk-associated substrate (CAS) and vinculin, were both independently shown to be crucial for theability of cells to transmit mechanical forces and to regulate cytoskeletal tension. Here, we identify a novel, direct binding interaction between CAS and vinculin. This interaction is mediated by the CAS SRC homology 3 domain and a proline-rich sequencein the hinge region of vinculin. We show that CAS localization in focal adhesions is partially dependent on vinculin, and that CAS-vinculin coupling is required for stretch-induced activation of CAS at the Y410 phosphorylation site. Moreover, CAS-vinculin binding significantly affects the dynamics of CAS and vinculin within focal adhesions as well as the size of focal adhesions. Finally, disruption of CAS binding to vinculin reduces cell stiffness and traction force generation. Taken to

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GC13-24851J" target="_blank" >GC13-24851J: Dissecting of BCAR1/CAS role in mechanosensing</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cellular and Molecular Life Sciences

ISSN

1420-682X

e-ISSN

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Volume of the periodical

71

Issue of the periodical within the volume

4

Country of publishing house

CH - SWITZERLAND

Number of pages

18

Pages from-to

727-744

UT code for WoS article

000330586500013

EID of the result in the Scopus database

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