Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10282018" target="_blank" >RIV/00216208:11310/14:10282018 - isvavai.cz</a>
Alternative codes found
RIV/00064165:_____/14:10282018
Result on the web
<a href="http://dx.doi.org/10.1016/j.jchromb.2014.07.023" target="_blank" >http://dx.doi.org/10.1016/j.jchromb.2014.07.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jchromb.2014.07.023" target="_blank" >10.1016/j.jchromb.2014.07.023</a>
Alternative languages
Result language
angličtina
Original language name
Direct silylation of Trypanosoma brucei metabolites in aqueous samples and their GC-MS/MS analysis
Original language description
A simple two-step method for the derivatization of polar compounds (lactate, alanine, glycerol, succinate and glucose) using hexamethyldisilazane (HMDS) and N,O-bis(trimethylsilyl)trifluoroacetamide (BSTFA) was developed. This method allows direct derivatization of aqueous samples wihout sample pretreatment. The method was used for the analysis of the metabolites of the unicellular organism Trypanosoma brucei. The limits of detection by GC-MS/MS analysis were in the range of 0.02 mgL(-1) for glucose to0.85 mgL(-1) for lactate.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences
ISSN
1570-0232
e-ISSN
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Volume of the periodical
967
Issue of the periodical within the volume
September
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
5
Pages from-to
134-138
UT code for WoS article
000341468600020
EID of the result in the Scopus database
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