Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283382" target="_blank" >RIV/00216208:11310/14:10283382 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/14:00441102
Result on the web
<a href="http://dx.doi.org/10.1098/rsta.2014.0199" target="_blank" >http://dx.doi.org/10.1098/rsta.2014.0199</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1098/rsta.2014.0199" target="_blank" >10.1098/rsta.2014.0199</a>
Alternative languages
Result language
angličtina
Original language name
Potential and limits of Raman spectroscopy for carotenoid detection in microorganisms: implications for astrobiology
Original language description
In this paper, it is demonstrated how Raman spectroscopy can be used to detect different carotenoids as possible biomarkers in various groups of microorganisms. The question which arose from previous studies concerns the level of unambiguity of discriminating carotenoids using common Raman microspectrometers. A series of laboratory-grown microorganisms of different taxonomic affiliation was investigated, such as halophilic heterotrophic bacteria, cyanobacteria, the anoxygenic phototrophs, the non-halophilic heterotrophs as well as eukaryotes (Ochrophyta, Rhodophyta and Chlorophyta). The data presented show that Raman spectroscopy is a suitable tool to assess the presence of carotenoids of these organisms in cultures. Comparison is made with the high-performance liquid chromatography approach of analysing pigments in extracts. Direct measurements on cultures provide fast and reliable identification of the pigments. Some of the carotenoids studied are proposed as tracers for halophiles,
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
DD - Geochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP210%2F10%2F0467" target="_blank" >GAP210/10/0467: Detection of Exobiological Markers Using Raman Spectroscopy as a Key Method</a><br>
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Philosophical Transactions of the Royal Society A: Mathematical, Physical and Engineering Sciences
ISSN
1364-503X
e-ISSN
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Volume of the periodical
372
Issue of the periodical within the volume
2030
Country of publishing house
GB - UNITED KINGDOM
Number of pages
17
Pages from-to
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UT code for WoS article
000344991900007
EID of the result in the Scopus database
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