Detection and quantitation of glutamate carboxypeptidase II in human blood
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F14%3A10283895" target="_blank" >RIV/00216208:11310/14:10283895 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/14:00428605 RIV/00064190:_____/14:#0000812 RIV/00216224:14110/14:00075573 RIV/65269705:_____/14:00061559
Result on the web
<a href="http://dx.doi.org/10.1002/pros.22796" target="_blank" >http://dx.doi.org/10.1002/pros.22796</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/pros.22796" target="_blank" >10.1002/pros.22796</a>
Alternative languages
Result language
angličtina
Original language name
Detection and quantitation of glutamate carboxypeptidase II in human blood
Original language description
BACKGROUND Glutamate carboxypeptidase II (GCPII) is a transmembrane enzyme that cleaves N-acetyl-L-aspartyl-L-glutamate (NAAG) in the brain. GCPII is highly expressed in the prostate and prostate cancer and might be associated with prostate cancer progression. Another exopeptidase, plasma glutamate carboxypeptidase (PGCP), was reported to be similar to GCPII and to share its NAAG-hydrolyzing activity. METHODS We performed a radioenzymatic assay with [H-3]NAAG as a substrate to detect and quantify the enzymatic activity of GCPII in plasma. Using a specific antibody raised against native GCPII (2G7), we immunoprecipitated GCPII from human plasma. We also cloned two PGCP constructs, expressed them in insect cells, and tested them for their NAAG-hydrolyzing activity. RESULTS We detected GCPII protein in human plasma and found that its concentration ranges between 1.3 and 17.2 ng/ml in volunteers not diagnosed with prostate cancer. Recombinant PGCP was enzymatically active but exhibited no
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GAP304%2F12%2F0847" target="_blank" >GAP304/12/0847: Glutamate Carboxypeptidase II and its role in cancer development</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2014
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Prostate
ISSN
0270-4137
e-ISSN
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Volume of the periodical
74
Issue of the periodical within the volume
7
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
768-780
UT code for WoS article
000334176000008
EID of the result in the Scopus database
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