Microbial abundance in surface ice on the Greenland Ice Sheet
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10296075" target="_blank" >RIV/00216208:11310/15:10296075 - isvavai.cz</a>
Alternative codes found
RIV/60076658:12310/15:43888908
Result on the web
<a href="http://dx.doi.org/10.3389/fmicb.2015.00225" target="_blank" >http://dx.doi.org/10.3389/fmicb.2015.00225</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3389/fmicb.2015.00225" target="_blank" >10.3389/fmicb.2015.00225</a>
Alternative languages
Result language
angličtina
Original language name
Microbial abundance in surface ice on the Greenland Ice Sheet
Original language description
Measuring microbial abundance in glacier ice and identifying its controls is essential for a better understanding and quantification of biogeochemical processes in glacial ecosystems. However, cell enumeration of glacier ice samples is challenging due totypically low cell numbers and the presence of interfering mineral particles.We quantified for the first time the abundance of microbial cells in surface ice from geographically distinct sites on the Greenland Ice Sheet (GrIS), using three enumeration methods: epifluorescence microscopy (EFM), flow cytometry (FCM), and quantitative polymerase chain reaction (qPCR).In addition, we reviewed published data on microbial abundance in glacier ice and tested the three methods on artificial ice samples of realistic cell (10(2)-10(7) cells ml(-1)) and mineral particle (0.1-100 mg ml(-1)) concentrations, simulating a range of glacial ice types, from clean subsurface ice to surface ice to sediment-laden basal ice. We then used multivariate statis
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/LM2010009" target="_blank" >LM2010009: CzechPolar ? Czech Polar Stations: Construction and management</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Frontiers in Microbiology
ISSN
1664-302X
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
MAR 24 2015
Country of publishing house
CH - SWITZERLAND
Number of pages
12
Pages from-to
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UT code for WoS article
000352518100001
EID of the result in the Scopus database
2-s2.0-84927523235