PIP2 and PIP3 interact with N-terminus region of TRPM4 channel

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10296261" target="_blank" >RIV/00216208:11310/15:10296261 - isvavai.cz</a>
Alternative codes found
RIV/67985823:_____/15:00446449 RIV/61388971:_____/15:00446449 RIV/61388963:_____/15:00446449 RIV/00216208:11130/15:10296261 RIV/00216208:11120/15:43909877
Result on the web
<a href="http://dx.doi.org/10.1016/j.bpc.2015.06.004" target="_blank" >http://dx.doi.org/10.1016/j.bpc.2015.06.004</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bpc.2015.06.004" target="_blank" >10.1016/j.bpc.2015.06.004</a>

Result language
angličtina
Original language name
PIP2 and PIP3 interact with N-terminus region of TRPM4 channel
Original language description
The transient receptor potential melastatin 4 (TRPM4) is a calcium-activated non-selective ion channel broadly expressed in a variety of tissues. Receptor has been identified as a crucial modulator of numerous calcium dependent mechanisms in the cell such as immune response, cardiac conduction, neurotransmission and insulin secretion. It is known that phosphoinositide lipids (PIPs) play a unique role in the regulation of TRP channel function. However the molecular mechanism of this process is still unknown. We characterized the binding site of PIP2 and its structural analogue PIP3 in the E733-W772 proximal region of the TRPM4 N-terminus via biophysical and molecular modeling methods. The specific positions R755 and R767 in this domain were identified as being important for interactions with PIP2/PIP3 ligands. Their mutations caused a partial loss of PIP2/PIP3 binding specificity. The interaction of PIP3 with TRPM4 channels has never been described before. These findings provide new insight into the ligand binding domains of the TRPM4 channel.
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
S - Specificky vyzkum na vysokych skolach

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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