Probing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297142" target="_blank" >RIV/00216208:11310/15:10297142 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1128/MCB.00448-1510.1128/MCB.00448-15" target="_blank" >http://dx.doi.org/10.1128/MCB.00448-1510.1128/MCB.00448-15</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1128/MCB.00448-15" target="_blank" >10.1128/MCB.00448-15</a>
Alternative languages
Result language
angličtina
Original language name
Probing the Biology of Giardia intestinalis Mitosomes Using In Vivo Enzymatic Tagging
Original language description
Giardia intestinalis parasites contain mitosomes, one of the simplest mitochondrion-related organelles. Strategies to identify the functions of mitosomes have been limited mainly to homology detection, which is not suitable for identifying species-specific proteins and their functions. An in vivo enzymatic tagging technique based on the Escherichia coli biotin ligase (BirA) has been introduced to G. intestinalis; this method allows for the compartment-specific biotinylation of a protein of interest. Known proteins involved in the mitosomal protein import were in vivo tagged, cross-linked, and used to copurify complexes from the outer and inner mitosomal membranes in a single step. New proteins were then identified by mass spectrometry. This approach enabled the identification of highly diverged mitosomal Tim44 (GiTim44), the first known component of the mitosomal inner membrane translocase (TIM). In addition, our subsequent bioinformatics searches returned novel diverged Tim44 paralogs
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Molecular and Cellular Biology
ISSN
0270-7306
e-ISSN
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Volume of the periodical
35
Issue of the periodical within the volume
16
Country of publishing house
US - UNITED STATES
Number of pages
11
Pages from-to
2864-2874
UT code for WoS article
000358279100013
EID of the result in the Scopus database
2-s2.0-84937861459