Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10297180" target="_blank" >RIV/00216208:11310/15:10297180 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1186/s13071-015-0765-x" target="_blank" >http://dx.doi.org/10.1186/s13071-015-0765-x</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s13071-015-0765-x" target="_blank" >10.1186/s13071-015-0765-x</a>

Result language
angličtina
Original language name
Xenodiagnosis of Leishmania donovani in BALB/c mice using Phlebotomus orientalis: a new laboratory model
Original language description
In areas of visceral leishmaniasis, the majority of infected hosts remain asymptomatic but potentially infectious. Their infectiousness for vectors is crucial for the transmission and can be quantified only by xenodiagnosis. However, in the case of humanhosts, xenodiagnosis is problematic. The BALB/c mouse model was designed to enable xenodiagnosis on VL hosts circumventing the need for human volunteers, it permits xenodiagnosis of the same individual host repeatedly, over several months. BALB/c mice were intradermally inoculated in the ear pinnae with Leishmania donovani, primarily metacyclic stages isolated from the thoracic midguts of experimentally-infected Phlebotomus orientalis females. Naive sand flies were allowed to feed on anaesthetized micein 1-3-weeks-interval, first on the site of inoculation of L. donovani (weeks 2-8 post infection, p.i.), later on the whole body of mice (weeks 9 - 15 p.i.). Infections of sand flies were evaluated microscopically or by PCR. Although inf
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EG - Zoology
OECD FORD branch
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Project
<a href="/en/project/GA13-07500S" target="_blank" >GA13-07500S: Vývoj a pohlavní rozmnožování Leishmania donovani v různých druzích flebotomů.</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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