Challenges of Flow-Cytometric Estimation of Nuclear Genome Size in Orchids, a Plant Group with Both Whole-Genome and Progressively Partial Endoreplication

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10311922" target="_blank" >RIV/00216208:11310/15:10311922 - isvavai.cz</a>
Alternative codes found
RIV/67985939:_____/15:00449202 RIV/67179843:_____/15:00449202 RIV/61389030:_____/15:00449202 RIV/60076658:12310/15:43889039 RIV/60076658:12220/15:43889039
Result on the web
<a href="http://dx.doi.org/10.1002/cyto.a.22681" target="_blank" >http://dx.doi.org/10.1002/cyto.a.22681</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/cyto.a.22681" target="_blank" >10.1002/cyto.a.22681</a>

Result language
angličtina
Original language name
Challenges of Flow-Cytometric Estimation of Nuclear Genome Size in Orchids, a Plant Group with Both Whole-Genome and Progressively Partial Endoreplication
Original language description
Nuclear genome size is an inherited quantitative trait of eukaryotic organisms with both practical and biological consequences. A detailed analysis of major families is a promising approach to fully understand the biological meaning of the extensive variation in genome size in plants. Although Orchidaceae accounts for similar to 10% of the angiosperm diversity, the knowledge of patterns and dynamics of their genome size is limited, in part due to difficulties in flow cytometric analyses. Cells in various somatic tissues of orchids undergo extensive endoreplication, either whole-genome or partial, and the G1-phase nuclei with 2C DNA amounts may be lacking, resulting in overestimated genome size values. Interpretation of DNA content histograms is particularly challenging in species with progressively partial endoreplication, in which the ratios between the positions of two neighboring DNA peaks are lower than two. In order to assess distributions of nuclear DNA amounts and identify tissu
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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