Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10312786" target="_blank" >RIV/00216208:11310/15:10312786 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00455588
Result on the web
<a href="http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1093706" target="_blank" >http://www.tandfonline.com/doi/full/10.1080/15384101.2015.1093706</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/15384101.2015.1093706" target="_blank" >10.1080/15384101.2015.1093706</a>

Result language
angličtina
Original language name
Longevity of U cells of differentiated yeast colonies grown on respiratory medium depends on active glycolysis
Original language description
Colonies of Saccharomyces cerevisiae laboratory strains pass through specific developmental phases when growing on solid respiratory medium. During entry into the so-called alkali phase, in which ammonia signaling is initiated, 2 prominent cell types are formed within the colonies: U cells in upper colony regions, which have a longevity phenotype and activate the expression of a large number of metabolic genes, and L cells in lower regions, which die more quickly and exhibit a starvation phenotype. Here, we performed a detailed analysis of the activities of enzymes of central carbon metabolism in lysates of both cell types and determined several fermentation end products, showing that previously reported expression differences are reflected in the different enzymatic capabilities of each cell type. Hence, U cells, despite being grown on respiratory medium, behave as fermenting cells, whereas L cells rely on respiratory metabolism and possess active gluconeogenesis. Using a spectrum of different inhibitors, we showed that glycolysis is essential for the formation, and particularly, the survival of U cells. We also showed that beta-1,3-glucans that are released from the cell walls of L cells are the most likely source of carbohydrates for U cells.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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