Catalytic enhancement of the heme-based oxygen-sensing phosphodiesterase EcDOS by hydrogen sulfide is caused by changes in heme coordination structure

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314048" target="_blank" >RIV/00216208:11310/15:10314048 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00455109
Result on the web
<a href="http://dx.doi.org/10.1007/s10534-015-9847-7" target="_blank" >http://dx.doi.org/10.1007/s10534-015-9847-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s10534-015-9847-7" target="_blank" >10.1007/s10534-015-9847-7</a>

Result language
angličtina
Original language name
Catalytic enhancement of the heme-based oxygen-sensing phosphodiesterase EcDOS by hydrogen sulfide is caused by changes in heme coordination structure
Original language description
EcDOS is a heme-based O-2-sensing phosphodiesterase in which O-2 binding to the heme iron complex in the N-terminal domain substantially enhances catalysis toward cyclic-di-GMP, which occurs in the C-terminal domain. Here, we found that hydrogen sulfideenhances the catalytic activity of full-length EcDOS, possibly owing to the admixture of 6-coordinated heme Fe(III)-SH- and Fe(II)-O-2 complexes generated during the reaction. Alanine substitution at Met95, the axial ligand for the heme Fe(II) complex, converted the heme Fe(III) complex into the heme Fe(III)-SH- complex, but the addition of Na2S did not further reduce it to the heme Fe(II) complex of the Met95Ala mutant, and no subsequent formation of the heme Fe(II)-O-2 complex was observed. In contrast, a Met95His mutant formed a stable heme Fe(II)-O-2 complex in response to the same treatment. An Arg97Glu mutant, containing a glutamate substitution at the amino acid that interacts with O-2 in the heme Fe(II)-O-2 complex, formed a sta
Czech name
—
Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
—

Project
<a href="/en/project/GA15-19883S" target="_blank" >GA15-19883S: Molecular mechanisms of intraprotein/interdomain signal transduction in model heme sensor proteins</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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