Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314751" target="_blank" >RIV/00216208:11310/15:10314751 - isvavai.cz</a>
Alternative codes found
RIV/61388963:_____/15:00449810 RIV/70883521:28110/15:43873020
Result on the web
<a href="http://dx.doi.org/10.1016/j.jtbi.2015.06.048" target="_blank" >http://dx.doi.org/10.1016/j.jtbi.2015.06.048</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jtbi.2015.06.048" target="_blank" >10.1016/j.jtbi.2015.06.048</a>

Result language
angličtina
Original language name
Enzymological description of multitemplate PCR-Shrinking amplification bias by optimizing the polymerase-template ratio
Original language description
Multitemplate polymerase chain reaction (PCR) is used for preparative and analytical applications in diagnostics and research. Classical PCR and qPCR are two basic setups with many possible experimental modifications. Classical PCR is a method of choiceto obtain enough material for subsequent sophisticated applications such as construction of libraries for next-generation sequencing or high-throughput screening. Sequencing and Single Nucleotide Primer Extension (SNuPE) employ one-strand synthesis and represent a distinct variant of analytical DNA synthesis. In all these applications, maintaining the initial ratio of templates and avoiding underestimation of minority templates is desired. Here, we demonstrate that different templates can amplify independently at low template concentrations (typical in qPCR setups, in which the polymerase concentration is usually several orders of magnitude higher than the template concentration). However, rare templates can be diluted in an effort to k
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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