Kinetic Analysis of a Globin-Coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Complex, Response Regulator, and Metal Cations on Autophosphorylation Activity

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314838" target="_blank" >RIV/00216208:11310/15:10314838 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1021/acs.biochem.5b00517" target="_blank" >http://dx.doi.org/10.1021/acs.biochem.5b00517</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1021/acs.biochem.5b00517" target="_blank" >10.1021/acs.biochem.5b00517</a>

Result language
angličtina
Original language name
Kinetic Analysis of a Globin-Coupled Histidine Kinase, AfGcHK: Effects of the Heme Iron Complex, Response Regulator, and Metal Cations on Autophosphorylation Activity
Original language description
The globin-coupled histidine kinase, AfGcHK, is a part of the two-component signal transduction system from the soil bacterium Anaeromyxobacter sp. Fw109-5. Activation of its sensor domain significantly increases its autophosphorylation activity, which targets the His183 residue of its functional domain. The phosphate group of phosphorylated AfGcHK is then transferred to the cognate response regulator. We investigated the effects of selected variables on the autophosphorylation reaction's kinetics. Thek(cat) values of the heme Fe(III)-OH-, Fe(III)-cyanide, Fe(III)-imidazole, and Fe(II)-O-2 bound active AfGcHK forms were 1.1-1.2 min(-1), and their K-m(ATP) values were 18.9-35.4 mu M. However, the active form bearing a CO-bound Fe(II) heme had a k(cat)of 1.0 min(-1) but a very high K-m(ATP) value of 357 mu M, suggesting that its active site structure differs strongly from the other active forms. The Fe(II) heme-bound inactive form had k(cat) and K-m(ATP) values of 0.4 min(-1) and 78 mu
Czech name
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Czech description
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Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Project
<a href="/en/project/GA15-19883S" target="_blank" >GA15-19883S: Molecular mechanisms of intraprotein/interdomain signal transduction in model heme sensor proteins</a><br>
Continuities
S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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