pREPORT: a multi-readout transcription reporter vector for fission yeast

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10314875" target="_blank" >RIV/00216208:11310/15:10314875 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/yea.3055" target="_blank" >http://dx.doi.org/10.1002/yea.3055</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/yea.3055" target="_blank" >10.1002/yea.3055</a>

Result language

angličtina

Original language name

pREPORT: a multi-readout transcription reporter vector for fission yeast

Original language description

Transcription factors are prominent regulators of gene expression that execute responses to various intracellular and extracellular stimuli. Recombinant transcription reporter systems can be conveniently used to study the DNA binding preferences and regulatory activity of a transcription factor under a range of conditions. Several reporter genes have been used to study transcription regulation in the fission yeast Schizosaccharomyces pombe. Each of these reporters has distinct advantages, such as high sensitivity or ease of use, and limitations, such as prohibitive costs or use of hazardous substances. To combine the strengths and mitigate the weaknesses of individual reporter genes, we have created pREPORT, a flexible multi-readout transcription reporter vector for fission yeast that employs an enhanced GFP-lacZ fusion and a customizable minimal promoter. With pREPORT, gene expression driven by the transcription factor of interest can be quantified in a number of ways, both in live ce

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/GPP305%2F12%2FP040" target="_blank" >GPP305/12/P040: Fission yeast CSL proteins in the maintenance of genome integrity</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Yeast

ISSN

0749-503X

e-ISSN

—

Volume of the periodical

32

Issue of the periodical within the volume

2

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

327-334

UT code for WoS article

000349022400002

EID of the result in the Scopus database

2-s2.0-84921712360