Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10319363" target="_blank" >RIV/00216208:11310/15:10319363 - isvavai.cz</a>
Alternative codes found
RIV/61388971:_____/15:00444555
Result on the web
<a href="http://dx.doi.org/10.1016/j.febslet.2014.12.023" target="_blank" >http://dx.doi.org/10.1016/j.febslet.2014.12.023</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.febslet.2014.12.023" target="_blank" >10.1016/j.febslet.2014.12.023</a>
Alternative languages
Result language
angličtina
Original language name
Interaction of Bordetella adenylate cyclase toxin with complement receptor 3 involves multivalent glycan binding
Original language description
The interaction of Bordetella pertussis adenylate cyclase toxin (CyaA) with complement receptor 3 (CR3, CD11b/CD18) involves N-linked oligosaccharide chains. To investigate the relative importance of the individual N-glycans of CR3 for toxin activity, the asparagine residues of the consensus N-glycosylation sites of CR3 were substituted with glutamine residues that cannot be glycosylated. Examination of CR3 mutant variants and mass spectrometry analysis of the N-glycosylation pattern of CR3 revealed that N-glycans located in the C-terminal part of the CD11b subunit are involved in binding and cytotoxic activity of CyaA. We suggest that these N-glycans form a defined clustered saccharide patch that enables multivalent contact of CR3 with CyaA, enhancingboth affinity and specificity of the integrin-toxin interaction. (C) 2014 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CE - Biochemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
FEBS Letters
ISSN
0014-5793
e-ISSN
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Volume of the periodical
589
Issue of the periodical within the volume
3
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
6
Pages from-to
374-379
UT code for WoS article
000349403300013
EID of the result in the Scopus database
2-s2.0-84921509836