Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F15%3A10319398" target="_blank" >RIV/00216208:11310/15:10319398 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/15:00083933 RIV/62156489:43210/15:43907697 RIV/00216305:26620/15:PU113301
Result on the web
<a href="http://dx.doi.org/10.1038/srep08868" target="_blank" >http://dx.doi.org/10.1038/srep08868</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/srep08868" target="_blank" >10.1038/srep08868</a>
Alternative languages
Result language
angličtina
Original language name
Paramagnetic Nanoparticles as a Platform for FRET-Based Sarcosine Picomolar Detection
Original language description
Herein, we describe an ultrasensitive specific biosensing system for detection of sarcosine as a potential biomarker of prostate carcinoma based on Forster resonance energy transfer (FRET). The FRET biosensor employs anti-sarcosine antibodies immobilized on paramagnetic nanoparticles surface for specific antigen binding. Successful binding of sarcosine leads to assembly of a sandwich construct composed of anti-sarcosine antibodies keeping the Forster distance (Ro) of FRET pair in required proximity. The detection is based on spectral overlap between gold-functionalized green fluorescent protein and antibodies@quantum dots bioconjugate (lambda(ex) 400 nm). The saturation curve of sarcosine based on FRET efficiency (F-604/F-510 ratio) was tested within linear dynamic range from 5 to 50 nM with detection limit down to 50 pM. Assembled biosensor was then successfully employed for sarcosine quantification in prostatic cell lines (PC3, 22Rv1, PNT1A), and urinary samples of prostate adenocarcinoma patients.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/ED1.1.00%2F02.0068" target="_blank" >ED1.1.00/02.0068: Central european institute of technology</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific Reports
ISSN
2045-2322
e-ISSN
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Volume of the periodical
5
Issue of the periodical within the volume
March 2015
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
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UT code for WoS article
000350549200018
EID of the result in the Scopus database
2-s2.0-84924406455