Absence of a conventional spindle mitotic checkpoint in the binucleated single-celled parasite Giardia intestinalis

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10325106" target="_blank" >RIV/00216208:11310/16:10325106 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11110/16:10325106

Result on the web

<a href="http://dx.doi.org/10.1016/j.ejcb.2016.07.003" target="_blank" >http://dx.doi.org/10.1016/j.ejcb.2016.07.003</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.ejcb.2016.07.003" target="_blank" >10.1016/j.ejcb.2016.07.003</a>

Result language

angličtina

Original language name

Absence of a conventional spindle mitotic checkpoint in the binucleated single-celled parasite Giardia intestinalis

Original language description

The spindle assembly checkpoint (SAC) joins the machinery of chromosome-to-spindle microtubule attachment with that of the cell cycle to prevent missegregation of chromosomes during mitosis. Although a functioning SAC has been verified in a limited number of organisms, it is regarded as an evolutionarily conserved safeguard mechanism. In this report, we focus on the existence of the SAC in a single-celled parasitic eukaryote, Giardia intestinalis. Giardia belongs to Excavata, a large and diverse supergroup of unicellular eukaryotes in which SAC control has been nearly unexplored. We show that Giardia cells with absent or defective mitotic spindles due to the inhibitory effects of microtubule poisons do not arrest in mitosis; instead, they divide without any delay, enter the subsequent cell cycle and even reduplicate DNA before dying. We identified a limited repertoire of kinetochore and SAC components in the Giardia genome, indicating that this parasite is ill equipped to halt mitosis before the onset of anaphase via SAC control of chromosome-spindle microtubule attachment. Finally, based on overexpression, we show that Giardia Mad2, a core SAC protein in other eukaryotes, localizes along intracytoplasmic portions of caudal flagellar axonemes, but never within nuclei, even in mitotic cells with blocked spindles, where the SAC should be active. These findings are consistent with the absence of a conventional SAC, known from yeast and metazoans, in the parasitic protist Giardia.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EA - Morphology and cytology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

European Journal of Cell Biology

ISSN

0171-9335

e-ISSN

—

Volume of the periodical

95

Issue of the periodical within the volume

10

Country of publishing house

DE - GERMANY

Number of pages

13

Pages from-to

355-367

UT code for WoS article

000385322700001

EID of the result in the Scopus database

2-s2.0-84990038871