Enantioselective separation of biologically active basic compounds in ultra-performance supercritical fluid chromatography
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10325127" target="_blank" >RIV/00216208:11310/16:10325127 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.aca.2016.04.044" target="_blank" >http://dx.doi.org/10.1016/j.aca.2016.04.044</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.aca.2016.04.044" target="_blank" >10.1016/j.aca.2016.04.044</a>
Alternative languages
Result language
angličtina
Original language name
Enantioselective separation of biologically active basic compounds in ultra-performance supercritical fluid chromatography
Original language description
The enantioseparation of basic compounds represent a challenging task in modern SFC. Therefore this work is focused on development and optimization of fast SFC methods suitable for enantioseparation of 27 biologically active basic compounds of various structures. The influences of the co-solvent type as well as different mobile phase additives on retention, enantioselectivity and enantioresolution were investigated. Obtained results confirmed that the mobile phase additives, especially bases (or the mixture of base and acid), improve peak shape and enhance enantioresolution. The best results were achieved with isopropylamine or the mixture of isopropylamine and trifluoroacetic acid as additives. In addition, the effect of temperature and back pressure were evaluated to optimize the enantioseparation process. The immobilized amylose-based chiral stationary phase, i.e. tris(3,5-dimethylphenylcarbamate) derivative of amylose proved to be useful tool for the enantioseparation of a broad spectrum of chiral bases. The chromatographic conditions that yielded baseline enantioseparations of all tested compounds were discovered. The presented work can serve as a guide for simplifying the method development for enantioseparation of basic racemates in SFC.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GP14-19278P" target="_blank" >GP14-19278P: Simultaneous dynamic complexation equilibria of analytes and buffer constituents with complex-forming agents in analytical separation systems</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytica Chimica Acta
ISSN
0003-2670
e-ISSN
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Volume of the periodical
932
Issue of the periodical within the volume
932
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
8
Pages from-to
98-105
UT code for WoS article
000377622600010
EID of the result in the Scopus database
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