Ploidy level and genome size variation in the homosporous ferns Botrychium s.l. (Ophioglossaceae)
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F16%3A10327092" target="_blank" >RIV/00216208:11310/16:10327092 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/s00606-016-1285-7" target="_blank" >http://dx.doi.org/10.1007/s00606-016-1285-7</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00606-016-1285-7" target="_blank" >10.1007/s00606-016-1285-7</a>
Alternative languages
Result language
angličtina
Original language name
Ploidy level and genome size variation in the homosporous ferns Botrychium s.l. (Ophioglossaceae)
Original language description
Recent cytological and molecular studies have investigated genome size variation and evolution in the homosporous ferns, but representatives of the Ophioglossaceae were largely overlooked, despite their evolutionary singularity. Flow cytometry analysis was performed on 41 individuals of eight species of the genera Botrychium (B.), Botrypus, and Sceptridium to estimate their ploidy level variation. In a subset of individuals, we also estimated the absolute genome size and corresponding C values. Additionally, a classical chromosome count was made on the recently described species B. alaskense. Ploidy level and new genome size records were determined for Botrychium alaskense, B. boreale, B. lanceolatum, B. "neolunaria" ined., B. pinnatum, Botrypus virginianus and Sceptridium multifidum. In addition, we confirmed the genome size of B. matricariifolium, B. minganense and B. lunaria. Two of the three major sub-clades of Botrychium differ slightly in their averaged homoploid genome size (subclade Lanceolatum, 24.72 +/- A 0.40 pg; subclade Lunaria, 27.51 +/- A 0.47 pg). Flow cytometry and chromosome counting confirmed that B. alaskense is a tetraploid. A new hexaploid cytotype, putatively formed through an autopolyploidization from the sympatric tetraploid cytotype, was detected in a single individual of B. boreale. This is only the second report of hexaploidy in the genus Botrychium and our data highlight the potential to find other ploidy levels within other Botrychium species. Interestingly, no difference within the monoploid genome sizes was detected between ploidy levels, thus supporting the hypothesis of genome size stability after polyploidization and rejecting the scenario of genome downsizing.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EF - Botany
OECD FORD branch
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Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Plant Systematics and Evolution
ISSN
0378-2697
e-ISSN
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Volume of the periodical
302
Issue of the periodical within the volume
5
Country of publishing house
AT - AUSTRIA
Number of pages
10
Pages from-to
575-584
UT code for WoS article
000374310400006
EID of the result in the Scopus database
2-s2.0-84960130917