Leishmania donovani development in Phlebotomus argentipes: comparison of promastigote- and amastigote-initiated infections

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10360289" target="_blank" >RIV/00216208:11310/17:10360289 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1017/S0031182016002067" target="_blank" >http://dx.doi.org/10.1017/S0031182016002067</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1017/S0031182016002067" target="_blank" >10.1017/S0031182016002067</a>

Result language

angličtina

Original language name

Leishmania donovani development in Phlebotomus argentipes: comparison of promastigote- and amastigote-initiated infections

Original language description

Leishmania parasites alternate in their life cycle between promastigote stages that develop in the gut of phlebotomine sand flies and amastigotes residing inside phagocytic cells of vertebrate hosts. For experimental infections of sand flies, promastigotes are frequently used as this way of infection is technically easier although ingestion of promastigotes by sand flies is unnatural. Here we aimed to answer a critical question, to what extent do promastigote-initiated experimental infections differ from those initiated with intracellular amastigotes. We performed side-by-side comparison of Leishmania development in Phlebotomus argentipes females infected alternatively with promastigotes from log-phase cultures or amastigotes grown ex vivo in macrophages. Early stage infections showed substantial differences in parasite load and representation of morphological forms. The differences disappeared along the maturation of infections; both groups developed heavy late-stage infections with colonization of the stomodeal valve, uniform representation of infective metacyclics and equal efficiency of transmission. The results showed that studies focusing on early phase of Leishmania development in sand flies should be initiated with intracellular amastigotes. However, the use of promastigote stages for sand fly infections does not alter significantly the final outcome of Leishmania donovani development in P. argentipes and their transmissibility to the vertebrate host.

Czech name

—

Czech description

—

Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10600 - Biological sciences

Project

<a href="/en/project/GA13-07500S" target="_blank" >GA13-07500S: Vývoj a pohlavní rozmnožování Leishmania donovani v různých druzích flebotomů.</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology

ISSN

0031-1820

e-ISSN

—

Volume of the periodical

144

Issue of the periodical within the volume

4

Country of publishing house

GB - UNITED KINGDOM

Number of pages

8

Pages from-to

403-410

UT code for WoS article

000398792200005

EID of the result in the Scopus database

—