Isatin Analysis Using Flow Injection Analysis with Amperometric Detection - Comparison of Tetrahedral Amorphous Carbon and Diamond Electrode Performance

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10367747" target="_blank" >RIV/00216208:11310/17:10367747 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1002/elan.201700272" target="_blank" >http://dx.doi.org/10.1002/elan.201700272</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elan.201700272" target="_blank" >10.1002/elan.201700272</a>

Result language

angličtina

Original language name

Isatin Analysis Using Flow Injection Analysis with Amperometric Detection - Comparison of Tetrahedral Amorphous Carbon and Diamond Electrode Performance

Original language description

Isatin is an endogenous indole compound in humans and rodents that has a wide range of biological activity. In rat models, isatin concentrations have been shown to increase in the heart, brain, blood plasma, and urine with stress. Studies on patients suffering from Parkinson&apos;s disease have indicated a correlation between progress of the disease and urinary output of the molecule. Isatin is electrochemically active and can therefore be detected with electrochemical techniques. In this work, we compared the performance of a nitrogen-incorporated tetrahedral amorphous carbon (ta-C:N) and a boron-doped nanocrystalline diamond thin-film electrode for the oxidative detection of this biomolecule using flow injection analysis with amperometric detection. The measurements were performed in 0.1 phosphate buffer pH 7.2. The ta-C:N electrode, like boron-doped nanocrystalline diamond, exhibits some excellent properties for electroanalytical measurements including (i) low background current and noise, (ii) microstructural stability at positive detection potentials, and (iii) good activity for a wide range of bioanalytes without conventional surface pretreatment. The results reveal that both electrodes exhibit a linear dynamic range from 100 to 0.1mol L-1, a short-term response variability 3-4% RSD (30 injections), a sensitivity of 18 mA M-1, and a limit of detection (S/N=3) of 1.0x10(-7)molL(-1) (14ngmL(-1) or 2.5fmol).

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electroanalysis

ISSN

1040-0397

e-ISSN

—

Volume of the periodical

29

Issue of the periodical within the volume

9

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

2147-2154

UT code for WoS article

000409516900018

EID of the result in the Scopus database

2-s2.0-85020451660