Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10367898" target="_blank" >RIV/00216208:11310/17:10367898 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1002/elps.201600547" target="_blank" >http://dx.doi.org/10.1002/elps.201600547</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/elps.201600547" target="_blank" >10.1002/elps.201600547</a>
Alternative languages
Result language
angličtina
Original language name
Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC
Original language description
Analysis of glycosylation is challenging due to micro- and macro-heterogeneity of the protein attachment. A combination of LC with MS/MS is one of the most powerful tools for glycopeptide analysis. In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin, and sex hormone-binding globulin was studied on a reversed phase chromatographic column. We observed reduction of the retention time with increasing number of monosaccharide units of glycans attached to the same peptide backbone. Fucosylation of larger glycans provides less significant retention time shift than for smaller ones. Retention times of glycopeptides were expressed as relative retention times. These relative retention times were used for calculation of upper and lower limits of glycopeptide retention time windows under the reversed phase conditions. We then demonstrated on the case of a glycopeptide of haptoglobin that the predicted retention time window boosts confidence of identification and minimizes false-positive identification. Relative retention time, as a qualitative parameter, is expected to improve LC-MS/MS characterization of glycopeptides.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Electrophoresis
ISSN
0173-0835
e-ISSN
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Volume of the periodical
38
Issue of the periodical within the volume
17
Country of publishing house
DE - GERMANY
Number of pages
7
Pages from-to
2193-2199
UT code for WoS article
000408987300012
EID of the result in the Scopus database
2-s2.0-85019210636