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Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F17%3A10367898" target="_blank" >RIV/00216208:11310/17:10367898 - isvavai.cz</a>

  • Result on the web

    <a href="http://dx.doi.org/10.1002/elps.201600547" target="_blank" >http://dx.doi.org/10.1002/elps.201600547</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1002/elps.201600547" target="_blank" >10.1002/elps.201600547</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Study of structure-dependent chromatographic behavior of glycopeptides using reversed phase nanoLC

  • Original language description

    Analysis of glycosylation is challenging due to micro- and macro-heterogeneity of the protein attachment. A combination of LC with MS/MS is one of the most powerful tools for glycopeptide analysis. In this work, we show the effect of various monosaccharide units on the retention time of glycopeptides. Retention behavior of several glycoforms of six peptides obtained from tryptic digest of haptoglobin, hemopexin, and sex hormone-binding globulin was studied on a reversed phase chromatographic column. We observed reduction of the retention time with increasing number of monosaccharide units of glycans attached to the same peptide backbone. Fucosylation of larger glycans provides less significant retention time shift than for smaller ones. Retention times of glycopeptides were expressed as relative retention times. These relative retention times were used for calculation of upper and lower limits of glycopeptide retention time windows under the reversed phase conditions. We then demonstrated on the case of a glycopeptide of haptoglobin that the predicted retention time window boosts confidence of identification and minimizes false-positive identification. Relative retention time, as a qualitative parameter, is expected to improve LC-MS/MS characterization of glycopeptides.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10406 - Analytical chemistry

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Electrophoresis

  • ISSN

    0173-0835

  • e-ISSN

  • Volume of the periodical

    38

  • Issue of the periodical within the volume

    17

  • Country of publishing house

    DE - GERMANY

  • Number of pages

    7

  • Pages from-to

    2193-2199

  • UT code for WoS article

    000408987300012

  • EID of the result in the Scopus database

    2-s2.0-85019210636