Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376228" target="_blank" >RIV/00216208:11310/18:10376228 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1126/sciadv.aar4536" target="_blank" >https://doi.org/10.1126/sciadv.aar4536</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1126/sciadv.aar4536" target="_blank" >10.1126/sciadv.aar4536</a>

Result language

angličtina

Original language name

Endocytosis-mediated siderophore uptake as a strategy for Fe acquisition in diatoms

Original language description

Phytoplankton growth is limited in vast oceanic regions by the low bioavailability of iron. Iron fertilization often results in diatom blooms, yet the physiological underpinnings for how diatoms survive in chronically iron-limited waters and outcompete other phytoplankton when iron becomes available are unresolved. We show that some diatoms can use siderophore-bound iron, and exhibit a species-specific recognition for siderophore types. In Phaeodactylum tricornutum, hydroxamate siderophores are taken up without previous reduction by a high-affinity mechanism that involves binding to the cell surface followed by endocytosis-mediated uptake and delivery to the chloroplast. The affinity recorded is the highest ever described for an iron transport system in any eukaryotic cell. Collectively, our observations suggest that there are likely a variety of iron uptake mechanisms in diatoms besides the well-established reductive mechanism. We show that iron starvation-induced protein 1 (ISIP1) plays an important role in the uptake of siderophores, and through bioinformatics analyses we deduce that this protein is largely diatom-specific. We quantify expression of ISIPI in the global ocean by querying the Tara Oceans atlas of eukaryotic genes and show a link between the abundance and distribution of diatom-associated ISIPI with ocean provinces defined by chronic iron starvation.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10600 - Biological sciences

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Science advances [online]

ISSN

2375-2548

e-ISSN

—

Volume of the periodical

4

Issue of the periodical within the volume

5

Country of publishing house

US - UNITED STATES

Number of pages

14

Pages from-to

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UT code for WoS article

000432440600028

EID of the result in the Scopus database

2-s2.0-85047167355