Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10376547" target="_blank" >RIV/00216208:11310/18:10376547 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00216-018-1098-3" target="_blank" >https://doi.org/10.1007/s00216-018-1098-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-018-1098-3" target="_blank" >10.1007/s00216-018-1098-3</a>

Result language
angličtina
Original language name
Using a portable Raman spectrometer to detect carotenoids of halophilic prokaryotes in synthetic inclusions in NaCl, KCl, and sulfates
Original language description
Cell suspensions of the haloarchaea Halorubrum sodomense and Halobacterium salinarum and the extremely halophilic bacterium Salinibacter ruber (Bacteroidetes) in saturated solutions of chlorides and sulfates (NaCl, KCl, MgSO4 center dot 7H(2)O, K2SO4, and (NH4)Al(SO4)(2)center dot 12H(2)O) were left to evaporate to produce micrometric inclusions in laboratory-grown crystals. Raman spectra of these pinkish inclusions were obtained using a handheld Raman spectrometer with green excitation (532 nm). This portable instrument does not include any microscopic tool. Acceptable Raman spectra of carotenoids were obtained in the range of 200-4000 cm(-1). This detection achievement was related to the mode of illumination and collection of scattered light as well as due to resonance Raman enhancement of carotenoid signals under green excitation. The position of diagnostic Raman carotenoid bands corresponds well to those specific carotenoids produced by a given halophile. To our best knowledge, this is the first study of carotenoids included in the laboratory in crystalline chlorides and sulfates, using a miniature portable Raman spectrometer.
Czech name
—
Czech description
—

Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10505 - Geology

Project
<a href="/en/project/GA17-04270S" target="_blank" >GA17-04270S: Distribution of biomarkers of extremophiles: learning about extreme environments on Earth to optimize exobiological research of Mars</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)