Hydrophilic interaction liquid chromatography in the separation of glycopeptides and their isomers
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10378562" target="_blank" >RIV/00216208:11310/18:10378562 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1007/s00216-018-1150-3" target="_blank" >https://doi.org/10.1007/s00216-018-1150-3</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00216-018-1150-3" target="_blank" >10.1007/s00216-018-1150-3</a>
Alternative languages
Result language
angličtina
Original language name
Hydrophilic interaction liquid chromatography in the separation of glycopeptides and their isomers
Original language description
The analysis of intact glycopeptides is a challenge because of the structural variety of the complex conjugates. In this work, we used separation involving hydrophilic interaction liquid chromatography using a superficially porous particle HALO (R) penta-HILIC column with tandem mass spectrometric detection for the analysis of N-glycopeptides of hemopexin. We tested the effect of the mobile phase composition on retention and separation of the glycopeptides. The results indicated that the retention of the glycopeptides was the combination of partitioning and adsorption processes. Under the optimized conditions, our HILIC method showed the ability to efficiently separate the glycoforms of the same peptide backbone including separation of the isobaric glycoforms. We achieved efficient separation of core and outer armlinked fucose of bi-antennary and tri-antennary glycoforms of the SWPAVGNCSSALR peptide and bi-antennary glycoform of the ALPQPQNVTSLLGCTH peptide, respectively. Moreover, we demonstrated the separation of antennary position of sialic acid linked via alpha 2-6 linkage of the monosialylated glycopeptides. Glycopeptide isomers are often differentially associated with various biological processes. Therefore, chromatographic separation of the species without the need for an extensive sample preparation appears attractive for their identification, characterization, and reliable quantification.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry
Result continuities
Project
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Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Analytical and Bioanalytical Chemistry
ISSN
1618-2642
e-ISSN
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Volume of the periodical
410
Issue of the periodical within the volume
20
Country of publishing house
DE - GERMANY
Number of pages
8
Pages from-to
5001-5008
UT code for WoS article
000438410800023
EID of the result in the Scopus database
2-s2.0-85047455098