Iron economy in Naegleria graben reflects its metabolic flexibility
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10381432" target="_blank" >RIV/00216208:11310/18:10381432 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1016/j.ijpara.2018.03.005" target="_blank" >https://doi.org/10.1016/j.ijpara.2018.03.005</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.ijpara.2018.03.005" target="_blank" >10.1016/j.ijpara.2018.03.005</a>
Alternative languages
Result language
angličtina
Original language name
Iron economy in Naegleria graben reflects its metabolic flexibility
Original language description
Naegleria gruberi is a free-living amoeba, closely related to the human pathogen Naegleria fowleri, the causative agent of the deadly human disease primary amoebic meningoencephalitis. Herein, we investigated the effect of iron limitation on different aspects of N. gruberi metabolism. Iron metabolism is among the most conserved pathways found in all eukaryotes. It includes the delivery, storage and utilisation of iron in many cell processes. Nevertheless, most of the iron metabolism pathways of N. gruberi are still not characterised, even though iron balance within the cell is crucial. We found a single homolog of ferritin in the N. gruberi genome and showed its localisation in the mitochondrion. Using comparative mass spectrometry, we identified 229 upregulated and 184 down-regulated proteins under iron-limited conditions. The most down-regulated protein under iron-limited conditions was hemerythrin, and a similar effect on the expression of hemerythrin was found in N. fowleri. Among the other down-regulated proteins were [FeFe]-hydrogenase and its maturase HydG and several heme-containing proteins. The activities of [FeFe]-hydrogenase, as well as alcohol dehydrogenase, were also decreased by iron deficiency. Our results indicate that N. gruberi is able to rearrange its metabolism according to iron availability, prioritising mitochondrial pathways. We hypothesise that the mitochondrion is the center for iron homeostasis in N. gruberi, with mitochondrially localised ferritin as a potential key component of this process. (C) 2018 Australian Society for Parasitology. Published by Elsevier Ltd. All rights reserved.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
International Journal for Parasitology
ISSN
0020-7519
e-ISSN
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Volume of the periodical
48
Issue of the periodical within the volume
9-10
Country of publishing house
GB - UNITED KINGDOM
Number of pages
9
Pages from-to
719-727
UT code for WoS article
000440960400005
EID of the result in the Scopus database
2-s2.0-85047194385