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Mitotic defects in fission yeast lipid metabolism 'cut' mutants are suppressed by ammonium chloride

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10383394" target="_blank" >RIV/00216208:11310/18:10383394 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1093/femsyr/foy064" target="_blank" >https://doi.org/10.1093/femsyr/foy064</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1093/femsyr/foy064" target="_blank" >10.1093/femsyr/foy064</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Mitotic defects in fission yeast lipid metabolism 'cut' mutants are suppressed by ammonium chloride

  • Original language description

    Fission yeast &apos;cut&apos; mutants show defects in temporal coordination of nuclear division with cytokinesis, resulting in aberrant mitosis and lethality. Among other causes, the &apos;cut&apos; phenotype can be triggered by genetic or chemical perturbation of lipid metabolism, supposedly resulting in shortage of membrane phospholipids and insufficient nuclear envelope expansion during anaphase. Interestingly, penetrance of the &apos;cut&apos; phenotype in mutants of the transcription factor cbf11 and acetyl-coenzyme A carboxylase cut6, both related to lipid metabolism, is highly dependent on growth media, although the specific nutrient(s) affecting &apos;cut&apos; occurrence is not known. In this study, we set out to identify the growth media component(s) responsible for &apos;cut&apos; phenotype suppression in Delta cbf11 and cut6-621 cells. We show that mitotic defects occur rapidly in Delta cbf11 cells upon shift from the minimal EMM medium (&apos;cut&apos; suppressing) to the complex YES medium (&apos;cut&apos; promoting). By growing cells in YES medium supplemented with individual EMM components, we identified ammonium chloride, an efficiently utilized nitrogen source, as a specific and potent suppressor of the &apos;cut&apos; phenotype in both Delta cbf11 and cut6-621. Furthermore, we found that ammonium chloride boosts lipid droplet formation in wild-type cells. Our findings suggest a possible involvement of nutrient-responsive signaling in &apos;cut&apos; suppression.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database

  • CEP classification

  • OECD FORD branch

    10600 - Biological sciences

Result continuities

  • Project

  • Continuities

    S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2018

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    FEMS Yeast Research

  • ISSN

    1567-1356

  • e-ISSN

  • Volume of the periodical

    18

  • Issue of the periodical within the volume

    6

  • Country of publishing house

    GB - UNITED KINGDOM

  • Number of pages

    7

  • Pages from-to

  • UT code for WoS article

    000449353000012

  • EID of the result in the Scopus database

    2-s2.0-85055955769