Determination of Indoxyl Sulphate on Carbon Film Composite Electrode and Carbon Paste Electrode

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10383431" target="_blank" >RIV/00216208:11310/18:10383431 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.2174/1573411013666170718095203" target="_blank" >https://doi.org/10.2174/1573411013666170718095203</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.2174/1573411013666170718095203" target="_blank" >10.2174/1573411013666170718095203</a>

Result language

angličtina

Original language name

Determination of Indoxyl Sulphate on Carbon Film Composite Electrode and Carbon Paste Electrode

Original language description

Background: This paper is focused on application of a recently developed disposable array of carbon composite electrodes and carbon paste electrode for the determination of indoxyl sulphate. Results: Measurement of basic electrochemical behaviour of indoxyl sulphate on working electrode resulted in the selection of BR buffer pH 2.0 and pH 3.0 as the optimum medium for carbon composite film electrode and carbon paste electrode, respectively. In this medium, concentration dependences of indoxyl sulphate were measured; calculated quantification limits were 0.72 mu mol L-1 for the carbon composite film electrode and 1.7 mu mol L-1 for the carbon paste electrode. Pasivation of the electrodes during repeated measurements and negligible indoxyl sulphate accumulation was observed. Applicability of the method for the indoxyl sulphate determination in spiked urine samples was tested in combination with the solid phase extraction, where methanol, BR buffer, deionised water and their combination were used as elution reagents. Conclusion: Selected graphite-based working electrodes were successfully applied for the development of suitable voltammetric method for the determination of indoxyl sulphate in BR buffer. Both electrodes showed similar behaviour and sufficient sensitivity and accuracy was reached; however, solid phase extraction was not successful due to ineffectual removal of interferents from human urine.

Czech name

—

Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

10406 - Analytical chemistry

Project

<a href="/en/project/GBP206%2F12%2FG151" target="_blank" >GBP206/12/G151: Center of novel approaches to bioanalysis and molecular diagnostics</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Current Analytical Chemistry

ISSN

1573-4110

e-ISSN

—

Volume of the periodical

14

Issue of the periodical within the volume

5

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

6

Pages from-to

530-535

UT code for WoS article

000446494800013

EID of the result in the Scopus database

2-s2.0-85053240617