Proteomic analysis of cardiac ventricles: baso-apical differences

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10384910" target="_blank" >RIV/00216208:11310/18:10384910 - isvavai.cz</a>

Alternative codes found

RIV/67985823:_____/18:00491342 RIV/00216208:11110/18:10384910

Result on the web

<a href="https://doi.org/10.1007/s11010-017-3266-8" target="_blank" >https://doi.org/10.1007/s11010-017-3266-8</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/s11010-017-3266-8" target="_blank" >10.1007/s11010-017-3266-8</a>

Result language

angličtina

Original language name

Proteomic analysis of cardiac ventricles: baso-apical differences

Original language description

The heart is characterized by a remarkable degree of heterogeneity. Since different cardiac pathologies affect different cardiac regions, it is important to understand molecular mechanisms by which these parts respond to pathological stimuli. In addition to already described left ventricular (LV)/right ventricular (RV) and transmural differences, possible baso-apical heterogeneity has to be taken into consideration. The aim of our study has been, therefore, to compare proteomes in the apical and basal parts of the rat RV and LV. Two-dimensional electrophoresis was used for the proteomic analysis. The major result of this study has revealed for the first time significant baso-apical differences in concentration of several proteins, both in the LV and RV. As far as the LV is concerned, five proteins had higher concentration in the apical compared to basal part of the ventricle. Three of them are mitochondrial and belong to the &quot;metabolism and energy pathways&quot; (myofibrillar creatine kinase M-type, l-lactate dehydrogenase, dihydrolipoamide dehydrogenase). Myosin light chain 3 is a contractile protein and HSP60 belongs to heat shock proteins. In the RV, higher concentration in the apical part was observed in two mitochondrial proteins (creatine kinase S-type and proton pumping NADH:ubiquinone oxidoreductase). The described changes were more pronounced in the LV, which is subjected to higher workload. However, in both chambers was the concentration of proteins markedly higher in the apical than that in basal part, which corresponds to the higher energetic demand and contractile activity of these segments of both ventricles.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Molecular and Cellular Biochemistry

ISSN

0300-8177

e-ISSN

—

Volume of the periodical

445

Issue of the periodical within the volume

1-2

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

211-219

UT code for WoS article

000437464800021

EID of the result in the Scopus database

2-s2.0-85040044865