Overcoming Volume Selectivity of Dipolar Recoupling in Biological Solid-State NMR Spectroscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10388242" target="_blank" >RIV/00216208:11310/18:10388242 - isvavai.cz</a>
Result on the web
<a href="https://doi.org/10.1002/anie.201805002" target="_blank" >https://doi.org/10.1002/anie.201805002</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/anie.201805002" target="_blank" >10.1002/anie.201805002</a>
Alternative languages
Result language
angličtina
Original language name
Overcoming Volume Selectivity of Dipolar Recoupling in Biological Solid-State NMR Spectroscopy
Original language description
Dipolar recoupling in solid-state NMR is an essential method for establishing correlations between nuclei that are close in space. In applications on protein samples, the traditional experiments like ramped and adiabatic DCP suffer from the fact that dipolar recoupling occurs only within a limited volume of the sample. This selection is dictated by the radiofrequency (rf) field inhomogeneity profile of the excitation solenoidal coil. We employ optimal control strategies to design dipolar recoupling sequences with substantially larger responsive volume and increased sensitivity. We show that it is essential to compensate for additional temporal modulations induced by sample rotation in a spatially inhomogeneous rf field. Such modulations interfere with the pulse sequence and decrease its performance. Using large-scale optimizations we developed pulse schemes for magnetization transfer from amide nitrogen to carbonyl (NCO) as well as aliphatic carbons (NCA). Our experiments yield a signal intensity increased by a factor of 1.5 and 2.0 for NCA and NCO transfers, respectively, compared to conventional ramped DCP sequences. Consistent results were obtained using several biological samples and NMR instruments.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10401 - Organic chemistry
Result continuities
Project
—
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Angewandte Chemie - International Edition
ISSN
1433-7851
e-ISSN
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Volume of the periodical
57
Issue of the periodical within the volume
44
Country of publishing house
DE - GERMANY
Number of pages
5
Pages from-to
14514-14518
UT code for WoS article
000448049800017
EID of the result in the Scopus database
2-s2.0-85055153569